NEW INSIGHTS IN THE REGULATION OF THE RECRUITMENT OF ΓΔ T LYMPHOCYTES BY HUMAN GLOMERULAR ENDOTHELIAL CELLS EXPOSED TO SHIGA TOXIN TYPE 2

DAVID ANTONIO ROSSO; FERNANDO DANIEL GOMEZ; NADIA MELILLO; MICAELA ROSATO; CRISTINA IBARRA; MARÍA MARTA AMARAL; CAROLINA CRISTINA JANCIC

Mar del Plata

Congreso; Reunion Anual de Sociedades de Investigación Clinica; 2022

Sociedad de Investigación clinica

Hemolytic uremic syndrome (HUS) is a late acute onset of symptoms that can occur after an intestinal infection with Shiga toxin(Stx)-producing Escherichia coli (STEC). STEC strains expressingStx2a are the most common etiologic pathogen responsible for severe cases of HUS in Argentina. The immune system participatesduring the development of HUS by promoting inflammation and exacerbating the renal tissue damage, initially caused by the Stx. γδT cells are a subset of T lymphocytes that act as early sensors ofcellular stress and infection. They infiltrate the tissues and, in kidney injury, γδ T cells can potentially be recruited as they expressthe fractalkine receptor (CX3CR1), which is involved in the homingof NK cells and monocytes in the kidney. Previously, we demonstrated that conditioned medium derived from Stx2a-treated human glomerular endothelial cells (HGEC), activated γδ T lymphocytesand induced a Th1-like profile. In this work, our aim was to evaluatewhether those conditioned media could modulate γδ T cell motility.To do this, we analyzed cell migration using the Transwell systemand F-actin polymerization in γδ T cells, by flow cytometry. For this,the cells were exposed to conditioned media derived from HGECtreated with Stx2a (0.01 ng/mL, 24 h) or untreated ones. As results,we found that Stx2a-treated HGEC conditioned medium increasedγδ T cell migration compared with culture medium alone (p