LEPTIN-THYROLIBERIN PATHWAY IN LEFT VENTRICLE HYPERTROPHY: DIRECT ACTION ON CARDIAC CELL CULTURE

LANDRO, MICAELA; AISICOVICH, MAIA; PERES DIAZ, LUDMILA SOLEDAD; RODRIGUEZ, MARIA FERNANDA; LANDA, MARÍA SILVINA; SCHUMAN, MARIANO LUIS; GARCÍA, SILVIA INÉS

Mar del Plata

Congreso; LXVIII Reunion Anual de la Sociedad Argentina de Investigacion Clinica; 2023

Sociedad Argentina de Investigacion Clinica

Cardiac TRH (cTRH) induces LVH and fibrosis in the heart. As the adiponectin Leptin induces TRH in the CNS, we hypothesized that in obesity, the increase of cTRH induced by hyperleptinemia was responsible for the LVH, mostly attributed to pressure load. In accordance, Agouti hyperleptinemic mice presented higher cTRH expression accompanied with a marked fibrosis and LVH. LVH and fibrosis were prevented with the cTRH system blockade pointing out that cTRH mediates LVH development induced by hyperleptinemia. To confirm that TRH´s Leptin induction was direct, we used cardiomyocytes cell line H9C2 stimulated with Leptin (100 ng/ml) and evaluated TRH expression by RT-PCR and peptide by WB. TRH expression and precursor content increased (p