FOLLOW-UP OF COMPARATIVE STUDY ON IMMUNE BARRIER DEVELOPMENT IN MAMMAL AND AVIAN MODELS: ASSESSING AGE, SECTION, AND DIET EFFECTS IN MICE AND QUAIL ENTEROIDS

JAIME, CRISTIAN E; MOINE, LUCIANA B; PIQUERAS, VIRGINIA; NAZAR, F. NICOLÁS; CORREA, SILVIA G

San Luis

Congreso; LXXI REUNIÓN CIENTÍFICA ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2023

Microbiota colonization initiates the development of the bacterial community in the digestive tract. In mammals, this process begins at birth and is subsequently influenced by lactation. In contrast, avian species start colonization through environmental microorganisms upon hatching. We have conducted a comparative study involving mammals and birds. Previously, we presented results on Japanese quail (Coturnix coturnix), evaluating various ages and diets (starter or adult food). Mucin levels displayed significant Age x Section interactions, while bursa cellularity was influenced by diet. Here, we examined C57BL/6 mice at 14, 21, 28, and 35 days old, divided into weaning and mixed-fed (mother-housed) groups on day 21. We investigated the effects of Age, Intestinal Section (Proximal vs. Distal), and Diet (Weaning vs. Mixed) on bacterial density, mucin production, and goblet cell frequency. Age influenced bacterial density/mg of feces in mother-housed animals (p≤0.05), with an Age x Diet interaction between 28 and 35 days (p