THE HIGH-FAT AND/OR HIGH-CARBOHYDRATE DIETS WORSENED THE BRAIN MICE SUSCEPTIBILITY TO DAMAGE PRODUCED BY ENTEROHEMORRHAGIC E. COLI (EHEC) SHIGA TOXIN 2

ARENAS-MOSQUERA, D; CERNY, N; CANGELOSI A; GEOGHEGAN PA; MALCHIODI, EL; DE MARZI, M; PINTO A.; GOLDSTEIN J.

Congreso; Reunion Anual de Sociedades de Biociencias; 2023

Shiga toxin 2 (Stx2), is responsible for producing hemolytic-uremic syndrome characterized by thrombocytopenia, microangiopathic hemolytic anemia and renal compromise, which primarily targets children up to 5 years of age. Encephalopathy produced by Stx2 is the mean predictor of death. Nutritional quality could be one of the reasons by which in the face of an EHEC outbreak, some patients experience more profoundly deleterious effects than others, including death. Thus, the aim of this study was to determine whether excessive high fat and/or high carbohydrate diets could negatively modulate the deleterious action of Stx2 on ventral anterior and ventral lateral thalamic nuclei, the neurological centers responsible for motor activity. Mice were fed with regular, high fat and/or high carbohydrate diets in the context of Stx2 or vehicle intravenous administrations to determine the alterations produced in the neurovascular unit at the cellular and functional levels. Statistical significance was performed using the One-way analysis of variance followed by Bonferroni post hoc test. The criterion for significance was p