Reductive environments potentiate IgG aggregation induced by copper(ii): therapeutic implications.

SAPORITO MAGRIÑÁ CRISTIAN; LOPEZ MONTAÑANA, L; PAGANO, G; RODRIGUEZ, A; TOPP N; DARCI A; FACIO ML; REPETTO MG

Congreso; Reunión conjunta SAIC. SAB, AAFE & AACyTAL 2023. LXVIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), XXV Reunión Anual de la Sociedad Argentina de Biología (SAB), LV Reunión Anual de la Asociación Argentina de Farmacología Expe; 2023

Sociedad Argentina de Investigacion Clinica

Free copper ions present in plasma react with H2O2 promoting oxidative damage. However, this metal also interacts with proteins causing misfolding and aggregation. Previously, we demonstrated that Cu(II) induces selective aggregation of IgG in human sera and pro-oxidant environments render these aggregates irreversible. Thus, it is possible that reducing environments decrease the pro-aggregating capacity of the metal by reducing it to Cu(I). The objectives of this research is to determine the effect of the reducing environment on Cu(II)-induced IgG aggregation. Human plasma (HP), purified protein (PP-IgG) and ascorbic acid (AA) solutions were employed, and optical density (OD), determination of proteins by Lowry, SDS-PAGE and silver staining were used. Cu(II) induces aggregation of PP-IgG (20 μM) determined by OD and increases the mass of aggregated proteins (p