Effect of hypercaloric diet and hypoandrogenism in rat lung

BIAGGIO VERONICA; ABALLAY FEDERICO; SALINAS MARYSOL; ZELARAYAN SARMIENTO, DANIELA; PIGUILLEM, SILVANA; CIMINARI, MARIA EUGENIA; RAZZETO, GABRIELA; SALINAS, ELOY; ALVAREZ, SILVINA; PEREZ CHACA, MARÍA VERONICA; GOMEZ, NIDIA NOEMI

Mar del Plata

Congreso; LXVIII Reunión Anual de Sociedad de Investigación Clínica; 2023

Obesity is a condition of oxidative stress and systemic inflammation that impairsrespiratory function. In addition, the presence of androgen receptors in lungindicates that testosterone play a key role in lung physiology. Obesity-inducedoxidative stress in adipose tissue is one of the main factors considered as a sourceof oxidants and inflammation mediator. The aim of this study was to analyse theeffects of androgen deficiency and obesity on lung morphophysiology. Male Wistarrats (200 ± 20 g) were divided in four groups: Control on normal diet (CoN),castrated on normal diet (KN), Control with hypercaloric diet (CoOB), and castratedwith hypercaloric diet (KOB) and they were sacrificed 30 days after castration.Biochemical parameters were analysed and the expression of antioxidantenzymes, NOX -2, FOXO, HO -1, and RA in lung. Histological sections wereobtained for morphometric analysis and Mason trichrome staining. ANOVA andTukey test were used for statistical analysis. Results demonstrated that TBARSlevels were increased in KOB (p< 0.001) and KN (p<0.01) groups. CAT activity wasincreased in the KN group (p<0.05). Expression of CAT decreased in the groupsCoOB and KN, and RA expression increased in KN group. Antioxidant enzymesNOX-2, SOD-2 (p<0.01) and GPx-1 (p<0.05) were increased in the KOB group.Morphometric analysis revealed large alveolar spaces, which may not have beenfunctional, in the CoOB and KOB groups (p<0.01). Manson trichrome stainingshowed an increase in connective tissue especially in KOB group. Morphometricanalysis revealed large alveolar spaces, possibly non-functional, in CoOB andKOB groups (p<0.01). We have previously demonstrated a condition of significantoxidative stress in castrated animal model. Obesity also produces a basicinflammatory situation. In our experimental model the lung present non-functionalalveolar spaces and increase in some regions connective tissue. Added to this,obesity would contribute to a pro-inflammatory state that would aggravate thedamage caused by androgen deficiency.