UP TO NO GOOD: ABERRANT GLYCOSYLATION AND SECRETORY IGA FUNCTION DURING INTESTINAL INFLAMMATION

CUTINE, AM.; CAGNONI, AJ.; MERLO, JP.; MOROSI, LG.; MANSELLE-COCCO, MN.; VEIGAS, F.; MARTINEZ ALLO, VC.; MORALES, RM.; GATTO, SG.; MAY, M.; RABINOVICH, GA.; TOSCANO, MA.; MARIÑO, KV.

Congreso; Reunion Anual de Biociencias 2022; 2022

SAIC-SAI

Ulcerative colitis (UC) is characterized by chronic and relapsing inflammation of the colon and has currently no cure. Secretory IgA (SIgA), a heavily glycosylated complex produced by plasma cells (PCs), plays a key role in maintaining gut immune homeostasis. In numerous inflammatory pathologies including UC, aberrant glycosylation affects the function of immune cells and glycoproteins; however, the specific role PCs and SIgA glycosylation in gut inflammation has not been characterized yet. Thus, we started by analyzing glycosylation-related genes in single cell RNA-sequencing data from UC patients’ biopsies. Notably, IgA+ PCs showed downregulation of genes involved in UDP-GlcNAc and CMP-sialic acid biosynthesis (UAP1, NANS) compared with controls (p