Exploring the role of the Galectin-1-glycan axis in an experimental model of colitis-associated colorectal cancer

MERLO, JP.; CAGNONI, AJ.; CUTINE, AM.; RABINOVICH, GA.; MARIÑO, KV.

Congreso; Reunion Anual de Biociencias 2021; 2021

SAIC-SAI

Objective. To understand the mechanisms underlying the protumorigenic role of Galectin-1 (Gal1) and associated glycans in colitis-associated colorectal cancer (CACRC).Materials & Methods. We performed transcriptomic analysis of tumor samples from WT and Gal1 KO mice in an experimental model of CACRC (azoxymethane/dextran sulfate sodium, AOM-DSS). Total RNA was extracted, purified, and sequenced. Sequencing data was pre-processed with FastQC and Trimmomatic, and pseudo-counts were estimated using Kallisto. Downstream analyses were run using R software v3.6. Immune infiltrate deconvolution was performed using MIXTURE with ImmuCC-seq signature. Enrichment analyses were performed using GSEAbase package. KO vs. WT comparisons were performed using Wilcoxon test.Results. We recently reported the immunosuppressive role of Gal1 in AOM-DSS through modulation of the CD8+ regulatory T cell compartment, with significantly decreased tumor number and volume in Gal1 KO mice. Here, we performed transcriptomics analysis in WT and Gal1 KO mice treated with AOM-DSS. Differential expression analysis and functional enrichment showed downregulation of angiogenesis-associated pathways (p