PESTICIDE IMIDACLOPRID ENHANCES MAMMARY EPITHELIAL CELL MOTILITY AND ALTERS MAMMARY BRANCHING MORPHOGENESIS

LEGUIZAMÓN MA; ALEJANDRO ESPAÑOL; BUJAN S; PONTILLO C; CHIAPPINI FLORENCIA; LASAGNA M; COCCA CLAUDIA; RANDI ANDREA; MIRET NOELIA

Congreso; LXVIII reunion anual de la Sociedad Argentina de Investigacion Clinica; 2023

The neonicotinoid imidacloprid (IMI) is a widely used pesticide, which binds to the nicotinic acetylcholine receptor (nAChR) and is an endocrine disruptor. As mammary gland development is regulated by hormones, we hypothesize that IMI exposure exerts alterations in the breast that favor tumorigenesis. We have reported that IMI boosts NMuMG mammary epithelial cell migration, metalloproteases (MMP)2 and 9 activity, and G protein-coupled estrogen receptor (GPER) expression. Herein, we aimed to delve into the IMI mechanism of action to promote NMuMG cell motility and to investigate whether it induces alterations in the mammary gland. NMuMG cells were treated with IMI (0.01-10 µM) for 24 h and the α7-nAChR protein levels were examined, finding an increment at 10 μM (190% p