MATERNAL INTAKE OF HIGH-FAT DIET DURING PREGNANCY AND LACTATION: SEX DIFFERENCES IN CARDIOMETABOLIC OUTCOMES IN OFFSPRING

DAMIAN SORIA; MELANIE OVIEDO; JUAN MANUEL GAETANI; ANALIA TOMAT; CRISTINA ARRANZ; ROSANA ELESGARAY; TAMARA ZAOBORNYJ; CAROLINA CANIFFI

Otro; Reunión conjunta SAIC SAI & FAIC 2022; 2022

The intrauterine environment associated with maternal high-fat diet (HFD) increases the risks of non-communicable diseases later in life. The aim is to evaluate the effects of maternal HFD on metabolic homeostasis and oxygen consumption in left ventricle (LV) in female (f) and male (m) offspring. Experimental design: Wistar f rats fed either HFD (60% calories from fat) or standard diet (SD) from pregnancy to offspring weaning. At 21 days of life, f and m offspring were weighted (body weight, BW, g), and systolic blood pressure (SBP, mmHg) and fasting glycemia (mg/dL) were measured. After sacrifice, retroperitoneal adipose tissue (rAT, g), soleous muscle (SM, g), and LV were removed and weighted. Also, tibial length (TL, cm) was measured. In LV, oxygen consumption was measured, and the respiratory control (RC) was calculated. The protocol was approved by CICUAL-FFyB-UBA. Results are expressed as mean±SEM. Statistical analysis: 2-way ANOVA, n=5-6 rats/group; interaction diet x sex (i): $p