INVOLVEMENT OF TOLL LIKE RECEPTOR 4 (TLR4) IN THE EFFECT OF HIGHFAT DIET AND/OR CHRONIC STRESS

SOFÍA QUIROGA; MARÍA PAULA MARCONE; MARÍA ROSA GONZALES MURANO; MARÍA AGUSTINA VIDAL; ANA MARÍA GENARO; ADRIANA LAURA BURGUEÑO

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

High fat diet (HFD) or chronic stress (CS) lead to low-grade inflammation, promoting neuroinflammation and memory impairment in rodents. To analyze the role of the inflammasome NLRP3 in these alterations we compared the effect of HFD and CS in C57Bl/6J (WT)and TLR4 KO mice. We previously found that HFD in WT increases body weight and basal glycemia levels. These results were not observed in KO mice. The aim of this study was to analyze the effect of HFD and/or CS on cognition and gene expression in hippocampus from WT and KO mice. At 4 weeks of age, males received standard diet (SD) or HFD. After 12 weeks, they were exposed (or not)to CS for 8 weeks, resulting in 4 groups for each strain: 1) SD, 2) SD+CS, 3) HFD, and 4) HFD+CS. To assess cognitive performance, we conducted the Object location test (OLT). Hippocampal mRNAexpression levels of PYCARD, NLRP3 (components of NLRP3 inflammasome) and NGF, BDNF and SIRT1 (as markers for cognitive deficits) were assess by qPCR. We found that OLT was altered inWT HFD or CS (p