ROLE OF NHE1 AND AQP2 IN BASAL AND ACIDOSIS- ASSOCIATED APOPTOSIS IN A MODEL OF RENAL CORTICAL COLLECTING TUBULE PRINCIPAL CELLS: MPKCCD

MAZZOCCHI MARINA; PORTA MICAELA; DI GIUSTO GISELA; PIZZONI ALEJANDRO; BELTRAMONE NATALIA; FORD PAULA; CAPURRO CLAUDIA; RIVAROLA VALERIA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAFIS

The association between growth mechanisms and intracellular pH(pHi) elicits the possibility that a chronic pH alteration modifies cellgrowth. Control of pHi is regulated in part by the isoform 1 of Na/Hexchanger (NHE1), an exchanger that, depending on the cell type,is involved in apoptotic or proliferative processes. We previouslyshowed in renal cells that the expression of aquaporin 2 water channel(AQP2) modifies growth. The aim of this study was to investigatewhether exposure of renal cells to acid medium altered apoptosisand, if so, its association with NHE1 and AQP2. To investigate this,we used a kidney principal cell line whose AQP2 expression canbe induced by arginine vasopressin (AVP): mpkCCDc14. Cell numbercounting, apoptosis and immunofluorescence studies were performed.Our studies show that after being cultured for 11 days inthe presence of AVP mpkCCDc14 cells increase their expression ofboth AQP2 and NHE1. AVP stimulated cells have a higher basalapoptosis than control ones (% apoptosis control 7.75±1.37 vs AVP13.33±1.85, p