ENDOTHELIN-1 IMPAIRS NOREPINEPHRINE NEURONAL TRANSPORTER IN PC12 CELLS. ROLE OF THE ENDOPLASMIC RETICULUM STRESS.

INNAMORATO COSTAS, FABIAN A; GIANGRECO, NICOLAS; ALVAREZ, GUADALUPE I.; COURREGES, ANA P.; MAROTTE, CLARISA; BIANCIOTTI, LILIANA G.; VATTA, MARCELO S.

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

Sociedad Argentina de Investigación Clínica

Neuronal norepinephrine transporter (NET) removes norepinephrine from the synaptic cleft. Several studies show that NET impairment is associated with cardiovascular diseases like hypertension (HT) whereas recent works link endoplasmic reticulum stress (ERE), triggered by misfolded proteins accumulation in the reticulum, with the development of HT. We recently reported that endothelin receptors (ETA/ETB) blockade in rats with salt-dependent HT decreases blood pressure and markers of ERE and ERE-dependent apoptosis as well as misfolded NET accumulation in the adrenal medulla. In the present study we aimed to unveil the mechanisms underlying these findings. PC12 cells were exposed to 10mM endothelin 1 (ET-1), 10nM thapsigargin (TG) or 0.1ug/ml tunicamycin (TN) (TG and TN were used as ERE positive controls) and NET and ERE markers expression were assessed by Western blot. Results were expressed as the mean ±SD, and p