INVESTIGADORES
SANCHEZ Marianela
congresos y reuniones científicas
Título:
Argentinean propolis associated to Larrea nitida Cav.exudate
Autor/es:
AGÜERO, M.B.; SVETAZ, L.; SÁNCHEZ, M.; LIMA, B.; ZACCHINO, S.; WUNDERLIN, D.; FERESIN, G.E.; TAPIA, A.
Reunión:
Congreso; XXVIII Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2010
Resumen:
We report the in vitro
antifungal activity of propolis extracts from the province
of San Juan (Argentina) as
well as the identification of their main antifungal compounds and botanical
origin.The antifungal activity was determined by the microdilution technique,
using reference microorganisms and clinical isolates. All dermatophytes and
yeasts tested were strongly inhibited by different propolis extracts and Larrea nitida exudate (MICs between 31.25 and 125
µg/mL). The most susceptible yeast was Cryptococcus
neoformans (31.25 µg/mL).The main antifungal compounds were
methyl-nordihydroguaiaretic acid (MNDGA) 1, nordihydroguaiaretic acid (NDGA) 2
and the MNDGA derivative 3. Both ligans 1, 2 displayed strong activity against
clinical isolates of Candidas spp, Cryptococcus spp, Trichophyton
rubrum and T. mentagrophytes
(MICs and MFCs between 31.25 and 62.25 µg /mL). Additionally, two epoxylignan were
isolated from propolis samples and Larrea
nitida exudates, showing moderate antifungal activity. This is the first
report matching the chemical profile of L.
nitida exudates with their corresponding propolis by HPLCMS, giving strong
evidences on the botanical origin of studied propolis from Argentina.