ROLE OF GLUTATHIONE S-TRANSFERASE GENETIC VARIANTS IN THE ONSET OF PORPHYRIA CUTANEA TARDA IN HIV INFECTED INDIVIDUALS

PAGNOTTA, PRISCILA; MELITO, VIVIANA; PARERA, VICTORIA; ROSSETTI, MARIA VICTORIA; BATLLE, ALCIRA; BUZALEH ANA MARIA; ZUCCOLI, JOHANNA

Milan

Congreso; ICPP 2019; 2019

Universidad de Milan

A high association between Porphyria Cutanea Tarda (PCT) and HIV infection (15%) is found in Argentina. Slight evidence exists about if PCT triggering in HIV patients is related to infection and/or therapy. Glutathione S-transferase (GST) is a member of Phase II drug metabolizing system. Some genetic variants that alter GST activity affecting xenobiotics levels are of clinical importance: GSTT1 null, GSTM1 null and GSTP1 (rs1695, c.313A>G). The aim was to analyze the possible role of these polymorphisms in PCT/HIV association.A population of Control (no PCT, no HIV), HIV (no PCT), PCT and PCT-HIV were studied. Analysis of GSTT1 null and GSTM1 null variants was performed by multiplex PCR. Genotyping of GSTP1 (rs1695, c.313A>G) variant was done by PCR-RFLP. For GSTT1, the homozygosis deletion frequencies were: 8.3% (Control), 6.7% (HIV), 10.5% (PCT), 14.3% (PCT-HIV); the frequency of null allele in PCT-HIV was higher than HIV group (p=0.075). For GSTM1, null genotype frequencies were: 41.7% (Control), 53.3% (HIV), 36.8% (PCT), 32.1% (PCT-HIV), showing a significant minor value (p