Effect of the coculture of porcine luteal cells during in vitro maturation on the dna methylation pattern of porcine blastocysts

TEPLITZ, G. M.; SIRARD, MA; LOMBARDO, DM.

Ciudad Autónoma de Buenos Aires

Jornada; VI Jornadas Internacionales Instituto de Investigación y Tecnología en Reproducción Animal INITRA; 2021

Facultad de Ciencias Veterinarias UBA

In pigs, the in vitro embryo development is still an inefficient biotechnology. The coculture with somatic cells is an alternative to improve suboptimal in vitro culture conditions. Aberrant DNA methylation patterns of genes required for development are common in in vitro produced embryos and may indicate carryover effects of suboptimal culture conditions on epigenetic signatures. In the present study, we investigated the potential effect of a coculture system of porcine luteal cells during in vitro maturation (IVM) on blastocyst development and DNA methylation of embryos using the EmbryoGENE DNA Methylation Array (EDMA). The cumulus-oocyte complexes were matured in vitro in TCM199 supplemented with human menopausal gonadotrophin (control) or in coculture with porcine luteal cells from passage 1 (PLC-1). The coculture with PLC-1 significantly increased the blastocyst rate (blastocyst/cleavage) (control: 14.4%; PLC-1 21.2%; Fisher´s test p