BECAS
CEPEDA Paula Julieta
congresos y reuniones científicas
Título:
PLASMA CELL-FREE DNA AS A POTENTIAL TOOL FOR OBSTETRIC OUTCOMES PREDICTION: QUANTIFICATION IN PREGNANT AND NONPREGNANT WOMEN
Autor/es:
CEPEDA, PAULA JULIETA; RACCA, MARÍA EMILIA; MILESI, MARÍA MERCEDES; VARAYOUD, JORGELINA; CARDOZO, MARÍA ALEJANDRA; LUQUE, ENRIQUE H; MUÑOZ-DE-TORO, MÓNICA; ROSSETTI, MARÍA FLORENCIA; RAMOS, JORGE GUILLERMO
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Anual de Sociedades de Biociencias. SAIC SAI&FAIC SAFIS; 2022
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
Cell-free DNA (cf-DNA) was associated with adverse obstetric outcomes prediction. We studied cf-DNA levels variability in plasma (pl-) samples to evaluate its utility as a potential biomarker. Pl-samples from first (1st), second (2nd) and third (3rd) trimester pregnant (P, N=59) and nonpregnant (NP, N=24) women were used. Cf-DNA was obtained using QIAmp DNA blood mini kit (QIAGEN). Actin-β (ACTB) gen was quantified by real-time quantitative PCR using an ACTB standard curve. For PCR assays, 1000 ACTB standard copies were added to samples as an internal control. Cf-DNA level was expressed as ACTB copy number and means±SD were statistically compared between groups. A correlation study between cf-DNA level and the gestational age (GA) at the delivery has been done. Cf-DNA increased in P respect to the NP group (2391±1388 vs 1506±446 ACTB copies, respectively; p
