CHRONOBIOLOGICAL STUDY OF PHYSIOLOGICAL VARIABLES AND ANTIOXIDANT METABOLISM IN Glycine max L.

PARDO SHEILA DANIELA; GATICA AGUILAR CAMILA; AZULOVICH ANA CECICLIA; NAVIGATORE FONZI LORENA; GOLINI REBECA SUSANA; PEREZ CHACA MARÍA VERÓNICA

San Luis

Otro; XXXIX Reunión Científica Anual Sociedad de Biología de Cuyo; 2021

Sociedad de Biología de cuyo

The rotation of the Earth on its axis exposes most of the planet's species to the daily oscillations of the environment. These oscillations constitute the Biological Rhythms, of which the circadian ones are the most studied. In plants, the initiation of photosynthesis after sunrise (periodic process) triggers the accumulation of metabolites derived from photosynthesis, including the production of reactive oxygen species (ROS). ROS represent a constant threat to plants, as they react with different cellular components generating oxidative stress. However, plants have developed mechanisms to maintain redox homeostasis. These oscillations in ROS production, removal and signaling involve the biological clock. The functions of clock oscillators in agriculturally important species such as soybean (Glycine max L.) are increasingly recognized. Soybean is one of the most important and cultivated legumes in the world and constitutes the main export item of Argentina, one of the main producing countries worldwide. Therefore, the objective of this work was to study the influence of the biological clock on the physiology and antioxidant metabolism of Glycine max L. in a 24-hour period. For this purpose, soybean cultures were grown in hydroponics for 7 days under standard conditions with a 14hs-light 10hs-dark photoperiod and biochemical (antioxidant enzyme activity, degree of lipid peroxidation and H2O2 levels), physiological (photosynthetic pigment concentration) and molecular (primer design, identification of transcription factor binding sites) parameters were determined with the leaves. Statistically significant differences were found in the activity of the antioxidant enzyme Catalase (p