TRPV4 CALCIUM CHANNEL IS INVOLVED IN AQUAPORIN 2-DEPENDENT RENAL CELL MIGRATION

BREUNIG A; RIVERA MF; GIOVANNINI G; CUTRERA N; BELTRAMONE N; RIVAROLA V; FORD P; CAPURRO C; DI GIUSTO G

Mar del Plata

Congreso; Reunion Conjunta SAIC SAI SAFIS; 2022

Sociedad Argentina de Fisiologia

Water channel Aquaporin-2 (AQP2) promotes renal cell migration. Considering that we have demonstrated a physical and functional interaction between AQP2 and Transient receptor potential vanilloid-4 channel (TRPV4), the aim of our work was to investigate the role of TRPV4 in AQP2-dependent renal cell migration. We used two renal cell models: one not expressing AQPs (WT) and another one expressing AQP2 (AQP2). TRPV4 involvement in cell migration was evaluated with wound healing and cell tracking assays in the presence of the selective inhibitor HC‐067047 (HC, 10 μM) and the specific agonist GSK1016790A (GSK, 10 nM). Cell viability was checked with trypan blue staining. TRPV4 localization was analyzed with immunofluorescence assays. Our results showed that both, inhibition and activation of TRPV4, reduced cell migration (%; WT: Control 22 ± 1, n=25; HC 22 ± 1, n=8, ns; GSK 19 ± 1, n=9, ns; AQP2: Control 30 ± 1, n=39; HC 25 ± 1, n=20, p