High fat diet induces coenzyme q10 depletion and dysregulation of mitochondrial complexes in rat liver cells

BÁEZ, FRANCISCO; SORIA, DAMIÁN ARIEL; CONTIN, MARIO; VALDEZ, LAURA; CANIFFI, CAROLINA; TRÍPODI, VALERIA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias; 2022

SAIC-SAFIS-SAI-FAIC

High-fat diet induces metabolic syndrome, oxidative stress, proinflammatory and prothrombotic state, hypertension, central obesity, and cardiovascular disease. Coenzyme Q (CoQ), is an endogenous potent antioxidant located in the mitochondria with a key role in the ATP production in the electron transport chain (ETC). Our aim is to assess the status of CoQ along with oxidized and reduced glutathione (GSSG and GSH), 2-thiobarbituric acid reactive substances (TBARS), mitochondrial complex activity (I-III and II-III), and serum lipidic profile in high-fat diet (HFD) fed rats. Wistar rats (n=20) were equally and randomly divided at weaning (21 days), into two groups that received HFD or control diet (CD) until 14 weeks of age. The diets used differ only in fat content (60% of total calories came from fat in HFD and 16% in CD). After sacrifice, plasmatic CoQ and TBARS and CoQ, GSSG and GSH in liver homogenate were chromatographycally determined together with the activity of liver mitochondrial complexes by spectrophotometry. In liver homogenate, CoQ and GSH were highly reduced in HFD respect to CD (CoQ: 0.7±0.2 vs 1.4±0.7uM and GSH: 38±3 vs 54±3µmol/mg protein, respectively, p