In-Vitro Exposure to Endocrine Disruptors Alters Inflammatory Markers in Whole Hypothalami and Immortalized GnRH Neurons

RIAÑO GÓMEZ JUAN; SORIANELLO, E; LIBERTUN C; LUX LANTOS V; FERNANDEZ MO

Congreso; ENDO 2021; 2021

ENDOCRINE SOCIETY

Bisphenol A (BPA), a monomer of polycarbonate plastics, and Benzophenones (BPs), used as UV-filters, are endocrine disrupting chemicals (EDC) found in everyday products. Previously, we showed that the in-vitro exposure to BPA decreased Kiss-induced GnRH expression in GN11 cells (donated by Dr. Susan Wray, NIH), immature GnRH neurons and that exposure to all the EDC decreased Kiss-induced GnRH gene expression in GT1-7 cells (donated by Dr. Pamela Mellon, UCSD), mature GnRH neurons. In this study, we analyzed the effect of in-vitro exposure to the same EDC (BPA, BP2 or BP3, Sigma, 1x10-9 M) or medium as control (C) in mature GnRH neurons (GT1-7 cells), and isolated hypothalami from adult Balb/c males on Glial fibrillary acidic protein (GFAP) and cytokine gene expression. Cells were exposed to the compounds for 12 or 24 h, in DMEM (high glucose) with charcoal-stripped FBS, and the hypothalami for 6 h to the EDC, in Krebs-Ringer buffer. After the incubations, RNA was extracted using Tri-Reagent (Molecular Research Center, OH, USA), 1-2 µg RNA was reverse transcribed and Real-Time PCR performed using specific primers. Results were expressed as Mean±SE and analyzed by T-test or ANOVA using Statistica (Statistica v StatSoft Inc, USA)Twenty-four hour BPA exposure increased il18 in GT1-7 cells (C=1.0±0.04, BPA=1.2±0.1, T-test p