Exposure to Endocrine Disruptors Alters Inflammatory Markers in Mouse Hypothalamus In-Vivo and In-Vitro

RIAÑO GÓMEZ JUAN; SORIANELLO, E; LIBERTUN C; LUX LANTOS V; FERNANDEZ MO

Buenos Aires

Congreso; . SETAC Latin América 14th Biennial Meeting; 2021

SETAC

IN-VITRO EXPOSURE TO ENDOCRINE DISRUPTORS ALTERS INFLAMMATORY MARKERS IN WHOLE HYPOTHALAMI AND IMMORTALIZED GNRH NEURONS Juan M Riaño Gómez1, Eleonora M Sorianello1, Carlos Libertun1,2, Victoria Lux-Lantos1 and Marina O Fernandez1. 1-Instituto de Biología y Medicina Experimental-CONICET, 2-Facultad de Medicina-UBA. Email: mfernandez@dna.uba.ar Bisphenol A (BPA) and Benzophenones (BPs) are endocrine disrupting chemicals (EDC). Previously, we showed that the in-vitro exposure to BPA, BP2 or BP3 decreased Kiss-induced GnRH gene expression in GT1-7 cells (Dr. Pamela Mellon, UCSD). In this study, we analyzed the in-vitro exposure of GT1-7 cells or isolated hypothalami from adult Balb/c males to BPA, BP2 or BP3 (Sigma, 1x10-9 M) on cytokine and glial fibrillary acidic protein (gfap) gene expression. After the incubations, RNA was extracted, reverse transcribed and qPCR performed using specific primers. Results were expressed as Mean±SE and analyzed by ANOVA using Statistica (Statistica v StatSoft Inc, USA). The in-vivo exposure to BP2 or BP3 (250 µg/kg/day, orally, for five days) effects on gene expression in C57Bl/6 mice was also analyzed. Animals were sacrificed, brains rapidly dissected and placed in dry ice, and kept -80 ºC until processed. AVPV and ARC micropunches were obtained and RNA was extracted, reverse transcribed and gene expression analyzed by qPCR. Twenty-four hour BPA increased il18 expression in GT1-7 cells (C=1.0±0.04, BPA=1.2±0.1, T-test p