Prostaglandin E2 promotes a unique resolutive profile in human macrophages

G. CABRERIZO; A. PALETTA; F. DI DIEGO GARCÍA; V. POLO; J. GEFFNER; A. CEBALLOS; F. REMES LENICOV

Mar del Plata

Conferencia; LXIX Reunión Científica de la Sociedad Argentina de Inmunología; 2022

Sociedad Argentina de Inmunología

Prostaglandin E2 (PGE2) is an immune mediator with recognized inflammatory properties, but also required for the initiation of the resolution process. Previously, we showed that PGE2 induced a pro-resolutive profile in macrophages. Alternative activation of macrophages with IL-4 is known to induce pro-resolutive features, although these can be reversed by inflammatory stimuli. Here, we aim to compare the actions of PGE2 with those of IL-4 in the acquisition of a resolutive profile by monocyte-derived macrophages.Human monocytes isolated from peripheral blood were cultured during 7 days in the presence of M-CSF (50 ng/ml). Macrophages were treated at day 5 of culture with IL-4 (20 ng/ml) or with PGE2 (1 M). Control macrophages were cultured without PGE2 or IL-4. Efferocytosis was quantified by incorporation of CFSE-stained apoptotic Jurkat cells and phenotype was analyzed by flow cytometry. Cytokines were measured by ELISA.PGE2 promoted efferocytosis by an average 15% increase of phagocytic macrophages (n=10, p