PERSONAL DE APOYO
BATTISTA Ariadna Gabriela
congresos y reuniones científicas
Título:
Extracellular ADP regulates lesion-induced cell proliferation and death in the zebrafish retina
Autor/es:
ARIADNA BATTISTA; PAULA FAILLACE
Reunión:
Conferencia; 3dr Latin American Stem Cell Network Symposium; 2011
Resumen:
Persistent neurogenesis in the adult teleost fish has
been a valuable tool to describe mechanisms involved in cell proliferation and
differentiation. Damage of the mature retina activates tissue regeneration from
intrinsic germinal cells.
The aim of this study was to investigate the role of
extracellular nucleotides on retinal regeneration.
Zebrafish were lesioned by intravitreal injections of
ouabain. Then, apyrase (enzyme that hydrolyses extracellular nucleotides) or
different antagonists of purinergic receptors were injected daily for 6 days in
the ouabain-treated eyes. On day 7 cell proliferation or death were determined.
We observed
that treatment with MRS2179, an antagonist of the ADP-activated P2Y1 receptor, completely blocked
lesion-induced increases in cell division. In contrast, cell proliferation was
not affected by blocking ADP-activated P2Y12, 13 receptors. Likewise, the
injection of an antagonist of adenosine P1 receptors or a mixture of
antagonists for ATP-activated P2Y11 or P2X1, 2, 3 receptors did not modify
lesion-induced cell division.
Additionally, extracellular nucleotides hydrolysis by treatment
with apyrase significantly increased cell death in injured retinas. This effect
was partially reproduced by blocking P2Y1 receptors.
The present study demonstrates a crucial role for
extracellular ADP in the repair of retina following injury via P2Y1 receptor.