Moderate zinc deficiency during fetal life and postnatal growth: effect on cardiac NO system. Pro-inflammatory cytokines and oxidative stress in male adult rats.

JURIOL LORENA; GOBETTO NATALIA; MENDES GARRIDO FACUNDO; PINEDA GONZALO; DASSO MARINA; TOBLLI J; CARRANZA MA; ELESGARAY ROSANA; TOMAT ANALÍA; ARRANZ CRISTINA

Congreso; International Congress in Translational Medicine, ?Cellular and Molecular Pathways as Therapeutic Targets?.; 2014

Moderate zinc deficiency during intrauterine and postnatal growth is a fetal programming model of hypertension, renal and cardiovascular dysfunction. Male zinc deficient rats showed reduced left ventricular (LV) wall thickness and ejection fraction. Aim: to evaluate, nitric oxide (NO) system, pro-inflammatory cytokines and oxidative state in LV of adult male rats exposed to this deficiency. Wistar rats received during pregnancy up to weaning low (L:8 ppm) or control (C:30 ppm) zinc diet. After weaning, male offspring fed low (l) or control (c) zinc diet during 60 days (Cc, Ll, Lc). At day 81, we evaluated in LV: Interleukin-6 and Tumor Necrosis Factor-α (IL-6, TNF-α), basal NO synthase (NOS) and endothelial (eNOS), neuronal (nNOS) and inducible (iNOS) isoforms activities; eNOS and Ser1177 eNOS phosphorylation protein expression (eNOS/β-actin, pSer1177eNOS/eNOS), mRNA expression (eNOS/GAPDH relative to Cc) and lipid peroxidation end products levels (TBARS). Values are means ± SEM, n=6/group. One way ANOVA , Bonferroni post-test. Cc Ll Lc TNF-α (immunohistochemistry;% of positive staining/area) 1.4±0.4 20.4±2.0* 1.7±0.3 IL-6 (immunohistochemistry;% of positive staining/area) 1.8±0.3 21.1±1.7* 2.8±0.8 Basal NOS Activity (pmol 14C L-Citrulline/g. tissue.min) 204±6 164±10* 157±11* pSer1177eNOS/eNOS (Western Blot; relative to Cc) 1.00±0.02 0.74±0.03* 1.02±0.16 TBARS (nmol/mg protein) 0.21±0.02 0.78±0.08* 0.47±0.02*? *p