GLYCOLYSIS, PENTOSE PHOSPHATE PATHWAY AND MEIOTIC PROGRESSION OF PORCINE OOCYTES DURING IN VITRO MATURATION

GABRIEL ÁLVAREZ; SEBASTIÁN CASIRÓ; SERGIO MORADO; GABRIEL DALVIT; PABLO CETICA

Chascomús

Jornada; XV Jornadas de la Sociedad Argentina de Biología; 2013

Sociedades de Biología de la República Argentina

We have previously observed the inhibition of glycolysis by sodium fluoride (NaF) and ATP and the inhibition of the pentose phosphate pathway by 6-aminonicotinamide (6-AN) and NADPH in porcine cumulus-oocytes complexes (COCs). Our aim was to determine the influence of these inhibitors on the meiotic progression of porcine oocytes during in vitro maturation. COCs were cultured during 44 h at 39ºC in medium 199 with gonadotropins (control) + 5 mM NaF, 10 mM ATP, 0.025 mM 6-AN or 0.125 mM NADPH. Oocyte nuclear stage was determined at 0, 24, 32, 40 and 44 h of maturation by Hoechst 33342 stain. Inhibition of glycolysis by NaF or ATP decreased the percentage of oocytes reaching metaphase II (P