GLUTATHIONE ROLE IN REACTIVE OXYGEN SPECIES PRODUCTION IN BOVINE OOCYTE MATURATION

SERGIO MORADO; PABLO CETICA; PAMELA STEIDEL; MARTHA BECONI; GABRIEL DALVIT

Chascomús

Jornada; XV Jornadas de la Sociedad Argentina de Biología; 2013

Sociedades de Biología de la República Argentina

Reduced glutathione (GSH) is a key antioxidant in normal cell function, involved in oocyte developmental competence. The aim of this work was to determine GSH role in reactive oxygen species (ROS) production during bovine oocyte in vitro maturation (MIV) and its aftereffect on in vitro fertilization (FIV). Cumulus-oocyte complexes (COCs) were obtained from slaughtered cow ovaries and matured in medium 199 (control) or supplemented with cysteine (GSH precursor) or chlorodinitro-bencene (CDNB, GSH removal) at 39°C, 5% CO2 for 22h. Nuclear maturation percentages were determined by the presence of metaphase II and FIV was determined by cleavage rate. ROS production was measured by DCHFDA at 0, 6, 12, 18 and 22h. COCs matured with cysteine showed a significant decrease in ROS levels at 12 and 18h (p