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MORAN MarÍa Celeste
congresos y reuniones científicas
Título:
MINTHOSTACHYS VERTICILLATA ESSENTIAL OIL ALONE OR MICROENCAPSULATED ACTIVATES TH1 CELLS AND INDUCES THE PRODUCTION OF OPSONIZING ANTIBODIES
Autor/es:
NOELIA ANAHÍ CAMPRA; MELINA VANESA MOLIVA ; SOFÍA ARSAUTE; JOSÉ MARÍA RAVIOLO ; NAHUEL MATÍAS; ALEJANDRO JAVIER PAREDES; MARÍA CELESTE MORAN; BELKYS MALETTO ; SANTIAGO DANIEL PALMA; SILVIA MARCELA ESTEIN ; ELINA BEATRIZ REINOSO; LAURA NOELIA CARIDDI
Lugar:
Buenos Aires
Reunión:
Otro; REUNIÓN DE SOCIEDADES DE BIOCIENCIAS 2020. LXVIII REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI); 2020
Institución organizadora:
SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC), SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) y SOCIEDAD ARGENTINA DE FISIOLOGÍA (SAFIS)
Resumen:
In a previous study, we demonstrated the adjuvant capacity of theMinthostachys verticillata essential oil (EO) (2.5 mg/ml) or microencapsulated essential oil (EOMC) (5 mg/ml) on the humoral andcellular immune response against Enterococcus faecium in Balb/cmice. The aim of this study was to evaluate the functionality of thespecific antibodies produced and to quantify the IFN-γ and IL-4cytokines. Balb/c mice (n=4 per group) were inoculated by subcutaneous injection (days 0, 14 and 28) as follows: Group 1: 100 µlsaline solution; Group 2: 100 µl bacterin+EO (2.5 mg/ml); Group 3:100 µl bacterin+ microcapsule wall material (2.5 mg/ml); Group 4:100 μl bacterin+EOMC (5 mg/ml). In all groups, bacterin was of E.faecium. Seven days after the last booster, blood was obtained andserum was separated. Mice were sacrificed by cervical dislocationand spleens were removed to obtain splenic lymphocytes for cellproliferation assay. To evaluate the functionality of the antibodies,the Opsonophagocytic Killing Assay was carried out using different dilutions of the sera (1/100, 1/200, 1/500, 1/1000, 1/2000), E. faecium (1x107 CFU/ml), bovine PMNs (1x107cells/ml) suspensionsand a source of bovine complement. Sera antibodies of mice fromGroup 4 mediated the opsonization of E. faecium and enhance thephagocytosis by PMNs up to the 1/500 dilution compared to Group2, which was up to the 1/200 dilution (p
