Cloning and expression of BoMyr during senescence and the effect of plant regulators on broccoli

GÓMEZ LOBATO, ME; CIVELLO, MARCOS; MARTÍNEZ, GUSTAVO

Puerto Madryn

Congreso; XLV Reunión anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2010

Broccoli (Brassica oleracea) is a vegetable belonging to the family of Cruciferae. It is harvested when their inflorescences are still immature, which causes considerable stress and triggers the senescence. Fresh broccoli contains glucosinolates witch are secondary metabolites. There is strong evidence that high consumption of brassica vegetables is associated with a decreased risk for several cancers, most likely due to their glucosinolate content. Glucosinolates co-exist with myrosinases, which are responsible for glucosinolate turn-over. When tissue damage due to herbivore occurs, glucosinolates are hydrolyzed by myrosinases into different degradation products witch play important roles, principally in plant interaction with pathogens and insect herbivores. In this work, it was cloned a fragment of Myrosinase (Myr), involved in the glucosinolates degradation. It was studied by RT-PCR the effect of senescence in different sections of broccoli and the effect of different plant regulators on the expression of Myr. Broccolis were treated with cytokine and ethylene, in order to delay or accelerate senescence, respectively. It was observed that, the expression of BoMyr was higher in section 1 (inflorescence) compared with the sections 2, 3, and leaves and the decreased significantly along the senescence treatment. In case of the effect of different plant regulators on the expression of Myr, it was observe that the expression decreased in ethylene treatment. However, treatment with cytokine increased significantly the expression at 24 h of treatment and then had similar expression that controls