STAPHYLOCOCCAL ENTEROTOXIN N PROMOTES PBMCs PROLIFERATION AND CYTOKINE RELEASE

DANIELA MARÍA REDOLFI ; SOFIA NOLI TRUANT ; MARIA BELÉN SARRATEA ; LAURA VALERIA IANNANTUONO LÓPEZ; MARIA BELÉN ANTONOGLOU ; EMILIO LUIS MALCHIODI ; MARISA MARIEL FERNANDEZ

Mar del Plata

Congreso; LXX Reunión de la Sociedad Argentina de Inmunología; 2022

Bacterial Superantigens (SAgs) are enterotoxins that interact simultaneously and in a non-classical way with the TCR V chain and the MHC-II. As this interaction occurs, it induces an exacerbated T cell proliferation and a massive cytokine release producing Toxic Shock Syndrome, among other affections.The present study aimed to analyze the capacity of Staphylococcal enterotoxin N (SEN), which belongs to the egc operon, to activate the immune system by evaluating its interaction with PBMCs from healthy donors. For this purpose, SEN was cloned in a prokaryotic plasmid, produced as a recombinant protein in E. coli and purified by Ni++/NTA column followed by Size Exclusion Chromatography. Further, SEN was identified by Mass Spectrometry. PBMCs from donors non-exposed to SAgs were incubated with different concentrations of SEN to evaluate proliferation in presence of [methyl-3H] thymidine. Supernatants were collected for quantitative analysis of IL-6, IL-12, IL-10, TNF and IFNusing ELISA. PBMCs were stimulated with 0.001-10 μg/ml of SEN and proliferated even when treated with as low as 0.001 μg/ml (p