EFFECT OF INFUSIONS WITH ANTIOXIDANT CAPACITY IN A FATTY LIVER DISEASE MODEL MADE WITH HEP-G2 CELLS

GUERRA L; GUALDIERI F; ROCHA GF; MONTALDO L

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias; 2022

Sociedad Argentina de Investigación Clínica

Non-Alcoholic Fatty Liver Disease (NAFLD) is associated with obesity; NAFLD treatment has lowefficiency. Our laboratory has demonstrated that antioxidant N-acetilcysteine could inhibitlipid accumulation on in vitro cell models; here we analyzed effects of plant infusion withantioxidant capacity (AC) on NAFLD model using Hep-G2 cells treated with 0.05mM Oleic Acid(OA) for 48 hs. We applied a Central Composite Rotatable Design to establish the extractionconditions that give infusions with maximum AC from Orange (Citrus sinensis, O) Carqueja(Baccharis articulate, C) and Quebracho (Schinopsis balansae, Q). These conditions were: 30mg dried leaves/mL, 70°C and 14 min. AC was determined by the DPPH radical method andFlavonoid content (FL) by the aluminum chloride method (standard solution: rutin). Hep-G2cells were treated for 48 hrs with OA and nontoxic dilutions of the infusions, non-treated Hep-G2 cells were considered controls. Intracellular neutral lipid content was evaluated using theOil Red O (ORO) technique, by quantification at 505nm and with an image-processing program(ImageJ). AC: 71.6 ± 2.2% (O), 51.5 ± 1.3% (C) and 60 ± 9.56% (Q); FL: 0.14 ± 0.04 mg/mL (O),0.44 ± 0.08 mg/mL (C) and 0.72 ± 0.24 mg/mL (Q) were observed. NAFLD cells show twice thelipid content than control (1.07 ± 0.14 AU [Control] vs 2.19 ± 0.32 AU [NAFLD], p