Cardiac kernel transcription factor NKX2-5 is regulated by a novel super-enhancer region in pluripotent stem cell-derived cardiomyocytes

SCARAFIA, MARÍA AGUSTINA; POSTEGUILLO, NICOLÁS; CASTAÑEDA, SHEILA; AMÍN, GUADALUPE; PALMA, MARIA BELEN; MÖBBS, ALAN M.; SMUCLER, JOAQUÍN; COLLI, CAROLINA; MORO, LUCÍA N.; WAISMAN, ARIEL; LA GRECA, ALEJANDRO; MIRIUKA, SANTIAGO

Mar del Plata

Congreso; Reunión Conjunta SAIC, SAI&FAIC, SAFIS 2022; 2022

The decisions to commit towards a cardiac cell fate are taken early during development. Defects in the cardiac program entail complications in cell specification and may lead to congenital heart diseases. Known as the cardiac kernel, TBX5, GATA4 and NKX2-5 are key players of the gene regulatory network governing cardiac differentiation. However, the mechanisms involved in their regulation and the decision making process of cardiac commitment are still not clear. RNA-seq results of human induced pluripotent stem cells (hiPSC) differentiated to cardiomyocytes (CM) revealed 2.208 regulated genes (p-adj