IMPACT OF MEDIUM PRESSURE HYPERBARIC OXYGENATION THERAPY ON THE CARDIOVASCULAR SYSTEM

PEIRONE, MAGDALENA; GUTIERREZ, CHRISTOPHER P. A.; CORIA, JAVIER; MIRANDA, SILVIA; DI GIROLAMO, GUILLERMO; CASTILLA, ROCÍO; BONAZZOLA, PATRICIA

Mar del Plata

Congreso; Reunión Anual de las Sociedades de Biociencia 2022.; 2022

Sociedad Argentina de Investigación Clínica

Medium Pressure Hyperbaric Oxygenation therapy (mHBOT) at 1.4 atm and 100% oxigen is recently used instead of the traditional HBOT at 2.4 atm. It is used as an adjunct therapy in burn wounds, diabetic foot ulcers and treatment of COVID-19 patients with respiratory distress. Up to date no studies were carried out to support the cardiovascular risk of both treatments. Thus, we studied the impact of mHBOT on the cardiovascular system in Sprague Dawley rats subjected to 30 sessions of 60 min at 1.4 atm and 100% O2. Isolated hearts were perfused and exposed to 30 min ischemia (I) and 45 min reperfusion (R) at 37ºC. Simultaneous mechanical and heat measurements and the infarcted area were evaluated. In aorta, histology and nitrite levels and the contractile response to noradrenaline (NA), acetylcholine (Ach) and nitroprusside (SNP) were studied. In plasma, sICAM and sEselectin were analyzed.Hearts from mHBOT-treated rats showed: 1) an increase in resting pressure during I (p