In-vitro effects of BPA, BP2 and BP3 on cell proliferation in mature GnRH neurons

JUAN M. RIAÑO GOMEZ; ELEONORA M. SORIANELLO; VICTORIA LUX-LANTOS; MARINA O. FERNANDEZ

Glasgow, Escocia

Congreso; Kisspeptin 4th World Conference 2022 - International Congress of Neuroendocrinology 2022; 2022

British Society of Neuroendocrinology, International Neuroendocrine Federation, Pan American Neuroendocrine Society

Previously we showed that the in-vitro exposure to BPA, BP2 and BP3, endocrine disruptors, (ED, 1x10-7 and 1x10-9 M, 24 hs) increasedcell proliferation in an immature GnRH cell line, GN11 cells (Susan Wray, USA). Kisspeptin also has a proliferative effect on these cells.The aim of this study is to evaluate the effects of the in-vitro exposure of the aforementioned compounds on cell proliferation in GT1-7cells (mature GnRH neurons, Pamela Mellon, UCSD, USA).Cell proliferation was evaluated using a Non-Radioactive Cell Proliferation Assay, MTS (Promega, WI, USA), after BPA, E2, BP2 andBP3 exposure (1x10-7 and 1x10-9 M, 12 or 24 hs). Participation of the nuclear estrogen receptors was evaluated using the estrogen receptorantagonist ICI 182780, (1x10-6 M). Effects of Kisspeptin (Kiss, 1x10-9 M) on cell proliferation was also evaluated. Results were recordedas Abs490/Abs490(Control), presented as Mean±SE and analyzed by Repeated measures ANOVA with a Fisher posttest (Statistica,StatSoft, OK, USA).Neither BPA nor BP2 modified cell proliferation (ANOVA ns, n=5). BP3 on the other hand increased cell proliferation compared to controlvalues both after 12 and 24 h exposure [12h-exposure: Control=1±0.08, BP3-9=1.27±0.12, BP3-7=1.39±0.13, BP2-9=1,04±0,05, BP2-7=1,01±0,06, BP3-7 different from Control p