Effects of Endocrine Disruptors on Autophagy Markers Gene Expression in Immortalized GnRH Neurons and Hypothalami of Adult Male Mice

JUAN MANUEL RIAÑO GÓMEZ; ELEONORA M. SORIANELLO; VICTORIA LUX-LANTOS; MARINA FERNANDEZ

Atlanta

Congreso; ENDO2022; 2022

Benzophenones (BP) and Bisphenol A (BPA) are endocrinedisrupting chemicals (EDC). Previously, we showed that the in-vitro exposure toEDC inhibited Kiss-induced gene expression in GT1-7 and GN11 cells, andaltered expression of inflammatory markers in GT1-7 cells and hypothalami ofadult male mice. In the present study, we evaluated the effects of the in-vivoexposure to BP2 and BP3 on the expression of autophagy markers inhypothalamic nuclei in C57Bl/6 adult male mice. Animals were sacrificed, brainsdissected and conserved -80ºC. Micropunches containing specific hypothalamicnuclei were obtained. Also, effects of the in-vitro exposure for 12 or 24 hours tothe ED were evaluated in mature (GT1-7) and immature (GN11) GnRH neurons.ARN was obtained, reverse-transcribed, and gene expression analyzed byqPCR. Results were presented as Media±SE and analyzed by ANOVA (Statistica,StatSoft Inc, USA). In-vivo, five-day exposure to BP2 or BP3 did not alterautophagy markers gene expression in the AVPV or ARC in adult male mice. InGT1-7 cells, Ulk1 was not significantly altered by any of the stimuli, whereas inGN11 cells BP3 significantly increased Ulk1 gene expression. We need moreexperiments to fully understand if exposure to ED alter the autophagy processin mouse hypothalamus. Keywords: Bisphenol A, Endocrine-DisruptingChemicals, Hypothalamus,