IN-VITRO EXPOSURE TO BISPHENOL A INCREASES GFAP GENE EXPRESSION IN WHOLE HYPOTHALAMI AND IL-18 GENE EXPRESSION IN GT1-7 CELLS

MARINA FERNANDEZ; CAMILA MARIGLIANO; JUAN MANUEL RIAÑO GÓMEZ; CARLOS LIBERTUN; VICTORIA LUX-LANTOS

CABA

Congreso; XX Jornada Anual de la Sociedad Argentina de Biología (SAB); 2018

Sociedad Argentina de Biología, Sociedad Uruguaya de Biología

Bisphenol A (BPA), a monomer of polycarbonate plastics, and Benzophenones (BPs), UV-filters, are endocrine disrupting chemicals (EDC) found in everyday products. In this study we analyzed the effects of the exposure to BPA, BP2 and BP3 on Glial fibrillary acidic protein (GFAP) and IL-18 (a pro-inflammatory cytokine expressed in GnRH neurons) in whole hypothalami isolated from adult Balb/c males and IL-18 in mature GnRH neurons (GT1-7 cells, donated by Dr. Pamela Mellon, UCSD). Hypothalami were incubated in Krebs-Ringer medium in the presence or absence of BPA, BP2 or BP3 (1x10-9M) or medium alone (C) for six hours. After the incubation, hypothalami were homogenized in Tri-Reagent (Molecular Research Center, OH, USA) and mRNA extracted for Gfap and Il-18 analysis by qPCR. GT1-7 cells were cultured in 12-well plates (200000 cells/well) in DMEM supplemented with 10% FBS, pyruvate and Penicillin/Streptomicin (complete medium). After 24 hours, media were changed to DMEM without phenol red, supplemented with 10% charcoal-stripped FBS, pyruvate and Penicillin/Streptomicin (stimulation medium). Cells were incubated with BPA, BP2, BP3 (1x10-9M) or vehicle (C) in stimulation media for 24 hours. RNA was extracted using Tri-Reagent and Il-18 analyzed by qPCR. RNA (1mg) was reverse transcribed and qPCR performed using specific primers. Hypothalami exposed to BPA showed increased Gfap gene expression (C=0.82±0.16, BPA=1.70±0.39; ANOVA p