Combination of baculovirus and flagellin as a vaccine formulation

CARRIQUIRIBORDE F; MOLINA G; MOLINARI P; TABOGA O; RUMBO M; BIEDMA ME

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias; 2022

SAIC.SAI-FAIC.SAFIS

Viral vectors are a relatively new vaccine platform that relies onrecombinant viruses to deliver selected immunogens into the host.Among them, Autographa californica multiple nuclear polyhedrosisbaculovirus (BV), categorized as a nonhuman viral vector with versatilefeatures, has been positioned as an excellent vaccine vector.The final goal of the project is to combine flagellin (FliC), a TLR-5ligand, with BV, in a single structure aiming to generate a synergisticimmune response. To try out this hypothesis, in this study we combineFliC and BV as a vaccine formulation and analysed both in vitroand in vivo immune responses. Using TLR5 reporter cell lines weobserved that FliC agonist activity is not impaired between 0-100MOI of BVs (p