Expression of L-arabinose isomerase by auto-induction in a batch biorreactor

DE SOUZA, TICIANE C; ALMEIDA, ANA CAROLINA; RABELO, MARÍA CRISTIANE; MANZO, RICARDO MARTÍN; MAMMARELLA, ENRIQUE J.; ROCHA BARRO GONÇALVES, LUCIANA

Búzios

Simposio; XXIII Simposio Nacional de Bioprocesos (SINAFERM), XIV Seminario de Hidrólisis Enzimática de Biomasas (SHEB) y XIV Seminario Brasilero de Tecnología Enzimática (ENZITEC); 2022

In the last years, changes in eating habits have promoted the generation of products that add multiplebenefits, medicinal or physiological, to the health of those who consume them. Among these products,D-tagatose stands out, a sweetener considered a good substitute for sucrose due to its similarity inflavor, with a low caloric content, high sweetness, in addition to not promoting residual taste in foods.D-tagatose can be obtained, by enzymatic route, through D-galactose isomerization reactions, catalyzed by L-arabinose isomerase (L-AI). However, in addition to the lack of commercial availabilityof L-AI, the high cost associated with the use of the conventional inducer, isopropyl β-D-1-thiogalactopyranoside (IPTG), in culture media, has made its production on a commercial scale unfeasible. Within this scenario, this study aims to present viable conditions for obtaining the enzymeL-arabinose isomerase from Enterococcus faecium DBFIQ E36 in Escherichia coli BL21 (DE3), in a benchtop bioreactor, using an alternative inducer, lactose, through the process of auto-induction.