LOW DOSES OF GLYPHOSATE-BASED HERBICIDE IN EWE LAMBS ALTER GENES INVOLVED IN FOLLICULAR DEVELOPMENT

ALEGRE AL; INGARAMO PI; ALARCÓN R; LOVERA L; DIOGUARDI G; RIVERA OE; MUÑOZ-DE-TORO M; LUQUE EH

Congreso; REUNION DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

The exposure to endocrine disruptor compounds (EDC) during critical periods of development may alter ovarian development. In previous studies, we demonstrated that glyphosate-based herbicides(GBH), acting as EDC, altered ovarian follicular development, in the ewe. In present study, we tested if the exposure of neonatal ewe lambs with the recently defined reference dose of GBH (US-EPA) alters the expression of genes involved in ovarian follicular development. Ewe lambs received oral treatment from postnatal day (PND) 1 to PND14 and the ovarian genes were evaluated at PND45. Ewe lambs were randomly assigned to the groups, GBH (n=5) received the reference dose (glyphosate at 1 mg/Kg/day) and controls (n=6) received saline solution. On PND45, the ovaries were collected and immediately frozen at -80 °C for further RNA extraction. Levels of mRNA of genes involved in follicular development such as steroid receptors (ESR1, ESR2, and PR), follicle-stimulating hormone receptor (FSHr), bone morphogenetic protein 15 (BMP15), Follistatin (FST), growth and differentiation factor 9 (GDF9), insulin-like growth factor 1 (IGF-1) and Activin A Receptor Type 2A (ACVR2) were determined. The mRNA expression of β-Actin was used ashousekeeping gene. The mRNA expression levels in GBH-treated lambs were indicated as values relative to those of the control group. Treated ewe lambs showed a significant reduction (p