Neutrophils, iron and microcystin: study of environmental toxic agents and oxidative stress in an in vitro model.

CHIESA, I; LAIRION F; CARBIA, C; SAPORITO MAGRIÑA C; REPETTO MG

Congreso; Reunión conjunta SAIC. SAI & FAIC, SAFIS 2022. LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), lXX Reunión Anual de la Sociedad Argentina de Inmunología (SAI) & 3er Congreso Franco-Argentino de Inmunología (FAIC), Reunión Anu; 2022

Human polymorphonuclear cells (PMN) are involved in the resolution of infectious and inflammatory processes, in the regulation of spontaneous tumorogenesis and in the development of cancer. Excess inorganic iron (Fe2+) and the presence of MC-LR/hepatotoxin (MC-LR) in drinking and recreational water are considered detrimental to human health and predispose to inflammatory and neoplastic processes associated with PMN-induced oxidative stress (OS). Therefore, our aim is to characterize the damage of both antigens by detecting OS and cellular respiratory burst in PMNs exposed to different concentrations of Fe2+ and MC-LR. Methodology: Enriched PMN fractions were obtained from venous blood of healthy volunteers to assess OS and cellular respiratory burst (RB). Each sample was separated into aliquots and incubated, one part with Fe2+ and the other with MC-LR, at 37 °C for 15 minutes. OS was assessed by measuring spontaneous chemiluminescence (CL) of the cells with a scintillation photon counter, and oxygen consumption (OC) with a Clark-type oxygen electrode at 37 °C, in basal condition or after activation (exposure to agonist for 15 minutes). In order to determine the effects of Fe2+ and MC-LR on PMN function, increasing concentrations were evaluated, Fe2+: 0, 6, 30, 300 µg/L and MC-LR: 0, 1, 25 µg/L. Results: The optimal cell concentration for assessing OS in PMNs was 8 x104 PMN/mL. OC decreased 40% with 6 and 30 µg Fe2+/L (p