OXDIDAIVE STRESS AND RESPIRATORY BURST STIMULATED BY URIDINE DIPHOSPHATE GLUCOSE (UDP?G) IN HUMAN NEUTROPHILS

CARBIA, C; CHIESA, I; LAIRION, F; LAZAROWSKI, A; REPETTO, MG

Congreso; SAIC. SAI & FAIC, SAFIS 2022. LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), lXX Reunión Anual de la Sociedad Argentina de Inmunología (SAI) & 3er Congreso Franco-Argentino de Inmunología (FAIC), Reunión Anual 2022 de la Soc; 2022

The purinergic agonist uridine diphosphate glucose (UDP-G) activates chemotaxis of human neutrophils (PMN). The aim of this study is to show that PMN activation by UDP-G is associated to oxidative stress (OS) and respiratory burst (RB). Methodology: OS was evaluated by measuring spontaneous chemiluminescence (CL) of cells with a scintillation photon counter, and RB by measuring oxygen consumption with an oxygen electrode type Clark at 37 ºC, at basal condition (control, C) or after activation (15 min) with lipopolysacharides (LPS, 2 µg/mL), phorbol myristate acetate (PMA, 20 ng/mL) or UDP-G (100 M). To establish the activation effect of the three agonists, the stimulation index (SI) was calculated for each different treatments as the ratio (quotient) between the mean of the parameter evaluated in activated cells and non-activated cells. To establish the effect of UDP-G on PMN function, increasing concentrations of UDP-G were evaluated (0-200 M). Results: The optimal cell concentration to evaluate OS in PMN was 1x105 PMN/mL with an enhancement in CL of 35% (p