Effect of ACTH on the preovulatory period in cattle and its influence on the expression of TGFB and its receptors in the ovary.?

PEUST C.; BARALE J.; BELOTTI, E.M.; ORTEGA, H.H.; SALVETTI, N.R.; HEIN, G.; MATILLER V.

Mar del Plata

Congreso; LXI Reunion Científica Anual de la Sociedad Argentina de Investigacion Clinica (SAIC).; 2018

SAIC

The components of the transforming growth factor-beta (TGFB)superfamily exert functions related to follicular development, ovulationand corpus luteum formation. We found an altered expressionof these components in the ovaries of animals with cystic ovariandisease (COD). In addition, dairy cattle are exposed daily to stressorsthat can affect reproductive function. Therefore, the aim of thisstudy was to determine the protein expression of the TGFB isoforms(TGFB1, 2, 3) and its receptors (TGFBR1, 2, 3) by immunohistochemistryin different follicular categories in granulosa and theca internacells from the ovaries during the preovulatory period of cattle.Animals were treated with adrenocorticotropic hormone (ACTH) every12 hours for five days before the ovulation. We detected a higherexpression of TGFB1 in granulosa cells of large preantral folliclesof the ACTH-treated group compared to those of the control group.TGFB2 and TGFB3 showed a higher expression in theca cells ofdominant follicles of the ACTH-treated group compared to antralfollicles of control group (p