Use of ultrasonography in preclinical Studies: tumor measurements using b-mode Ultrasound real time, and vascular characterization By power doppler ultrasound of Tumors in immunodeficient mice.

BELOTTI, E.M.; DÍAZ, P.; STASSI A.; SALINAS F.; SALVETTI, N.R.; MARELLI, B.E.; PODESTÁ, E.; ORTEGA, H.H.

Mar del Plata

Congreso; V CONGRESO NACIONAL DE LA ASOCIACIÓN ARGENTINA DE CIENCIA Y TECNOLOGÍA DE ANIMALES DE LABORATORIO (AACYTAL); 2016

AACyTAL

The standard method for measurement of subcutaneous tumorsin immunodeficient mice is through the use of calipers. 􀀰evertheless,it introduces a lot of bias. 􀀫n the last decades, the use ofultrasonography as a diagnosis method for tumors has advanced,including the possibility of getting better assessment to gaugethose neoplasms of different 􀁙ays. 􀀷ltrasound 􀀊􀀷S􀀋 po􀁙er 􀀦opplerimage is 􀁙idely applied for observing the perfusion of vasculat urebecause it provides noninvasive and reproducible information andhelps in assessing longitudinally the angiogenesis􀀏related diseaseas 􀁙ell as monitoring the therapeutic response. The objectiveof this study is to report a different option to evaluate tumors inpreclinical procedures. 􀀫mmunodeficient mice 􀀊Foxn􀀓 nu/nu 􀀤alb/cathymic nude mice􀀋 injected 􀁙ith neoplastic cells 􀀊􀀯􀀦A􀀏􀀯􀀤􀀏􀀔􀀕􀀓􀀋,on the right flan􀁍, 􀁙ere evaluated every 􀁙ee􀁍 during 􀀔 months,beginning at 􀀓􀀖 days of the injection of neoplastic cells􀀝 they 􀁙ereassessed by 􀀷S 􀁙ith a color 􀀦oppler equipment 􀀊􀀼􀀘 􀀸et, 􀀯indray,China􀀋, coupled to a 􀀗.􀀒􀀏􀀓􀀒 􀀯Hz linear probe 􀀊􀀙􀀗L􀀗􀀒EA􀀸, 􀀯indray􀀋. 􀀤􀀏mode scanning in real time 􀁙as used, for different mensuration􀀊conjugated diameter and volume􀀋 of the tumors. The useof 􀀷S equipment equipped 􀁙ith 􀀦oppler allo􀁙s us to add datato the morphometric usually acquired 􀁙ith standard ultrasoundequipment, li􀁍e blood flo􀁙 intensity of the tumor at different momentsin the protocol. These parameters allo􀁙 us to get moremeasures than caliper mensuration, in less time of manipulation ofthe animals 􀀊the acquisition duration of each po􀁙er 􀀦oppler image􀁙as about 􀀚􀅌􀀓􀀒 seconds􀀋, 􀁙ith a non􀀏invasive method, providingmore objective data. The use of tools to provide as many data aspossible 􀁙ith the least handling of animals is a brea􀁍through thatinvolves the refinement of animal procedures and contributes tothe 􀁙elfare of the experimental animals.