ENDOCANNABINOID SYSTEM, TRANSPORT OF NUTRIENTS AND CELL DEATH IN PLACENTAS-DERIVED FETAL GROWTH RESTRICTION.

AISEMBERG, JULIETA; MARVALDI, CAROLINA; WOLFSON, MANUEL LUIS; FRANCHI, ANA MARIA

Mar del Plata

Congreso; Reunion anual de sociedades de biociencias; 2022

Sociedad Argentina de Investigación Clinica

The endocannabinoid system (ECS) is a lipid signaling system that includes endogenous ligands (AEA, 2-AG), receptors (CB1, CB2, TRPV1), and biosynthetic (NAPE-PLD) and hydrolysing (FAAH) machineries. Its role has become significantly important to multiple pregnancy events, including implantation and placentation. Alterations in placental ECS may impair pregnancy success.The aim of the current study was to use a mice model of dexamethasone-induced fetal growth restriction (FGR) in order to investigate whether ECS alterations in the placenta might be occurring. Moreover, as FGR can occur via impaired transport of key nutrients to the fetus, we further characterized the effects of dexamethasone on the expression on the placental glucose transporter 1 (GLUT1). Given that placental insufficiency results in FGR associated with placental apoptosis, we sought to investigate apoptosis in these placentas. Pregnant mice received a subcutaneous injection of vehicle or dexamethasone (8 mg/kg) from gestational day 14 to 15. Dam subsets were euthanised at day 14 to 16 of pregnancy for placenta assessment via western blot, qPCR and FAAH activity. The results were analyzed with a one-way ANOVA and Tukey test (p