UP TO NO GOOD: ABERRANT GLYCOSYLATION AND SECRETORY IGA FUNCTION DURING INTESTINAL INFLAMMATION

ANABELA M. CUTINE; ALEJANDRO J. CAGNONI; JOAQUÍN MERLO; LUCIANO G. MOROSI; MONTANA N. MANSELLE COCCO; FLORENCIA VEIGAS; VERÓNICA MARTÍNEZ ALLO; ROSA MORALES; SABRINA G. GATTO; MARÍA MAY; GABRIEL A RABINOVICH; MARTA A. TOSCANO; KARINA V. MARIÑO

Mar del Plata

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

SAIC SAI&FAIC SAFIS

Ulcerative colitis (UC) is characterized by chronic and relapsing inflammation of the colon and has currently no cure. Secretory IgA (SIgA), a heavily glycosylated complex produced by plasma cells (PCs), plays a key role in maintaining gut immune homeostasis. In numerous inflammatory pathologies including UC, aberrant glycosylation affects the function of immune cells and glycoproteins; however, the specific role PCs and SIgA glycosylation in gut inflammation has not been characterized yet. Thus, we started by analyzing glycosylation-related genes in single cell RNA-sequencing data from UC patients’ biopsies. Notably, IgA+ PCs showed downregulation of genes involved in UDP-GlcNAc and CMP-sialic acid biosynthesis (UAP1, NANS) compared with controls (p