Impaired ovarian response to exogenous gonadotropins in female mice offspring born to mothers prenatally exposed to Bisphenol A (BPA), Benzophenone-3 (BP3), or its combination (BPA+BP3).

GALLIANI, VALENTINA; ABUD, JULIÁN; FISCHER, FLORENCE; KRETSCHMER, TOBIAS; ZENCLUSSEN AC; ZENCLUSSEN, MARÍA LAURA; RODRÍGUEZ HORACIO ADOLFO*

Mar del Plata

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC), LXIX Reunión Anual de la Soc. Argentina de Inmunología (SAI); 2022

Sociedad Argentina de Investigación Clínica

BP3 belongs to organic sunscreens used in personal care productsto help minimize the damaging effects of ultraviolet (UV) radiation.BPA is used in the production of polycarbonate plastics and epoxyresins. Several studies have evidenced that both chemicals canaffect reproductive parameters by endocrine disruption, but thereare no studies assessing the potential toxicity of their combinedexposure. In this study we analyze if ovary´s ability to respond tostimulation with gonadotropins is affected by prenatal exposure toBP3, BPA or BP3+BPA. BP3 dissolved in olive oil was dermallyapplied whereas oral administration was used for BPA dissolved inethanol-water (0.001%). Chemicals were administered to C57BL/6pregnant females mated with BALB/c males according to the followingscheme: a) BP3 (50 mg/kg/day); b) Control (olive oil and ethanol-water); c) BPA( 4μg/kg/day); d) BPA+BP3. Dams were exposedfrom gestation day 0 (gd0) to gd10 in two successive pregnancies.In both pregnancies we recorded the weight of the dams and of thelitters from postnatal day 0 (PND0) to PND21, gestations duration,pups per mother and sex ratio. Besides, female offspring were injectedwith PMSG at PND27 and hCG was injected 48 hours later.Oocyte count was performed 14hs after hCG injection. Duration ofgestation, pups per mother, sex ratio and weights of offspring weresimilar between treated groups and control in both pregnancies.Interestingly, we observed a significant decrease in the number ofovulated oocytes of BPA-exposed female offspring of first pregnancyand of the three groups (BPA, BP-3, BP-3/BPA) in the offspringof the second pregnancy. Besides, we can conclude that animalsprenatally exposed to BP3, BPA or their combination impaired theability of the ovary to respond to gonadotropins stimulation