MAGNESIUM SULFATE RESTORES AQP9 EXPRESSION AND FUNCTION IN HUMAN TROPHOBLAST EXPOSED TO NITRATIVE STRESS

SIERRA MATÍAS N; MEDINA YOLLYSETH; ANUD CAROLINA; REPPETTI JULIETA; MARTÍNEZ NORA; DAMIANO ALICIA

Simposio; 9th Latin American Symposium on Maternal-Fetal Interaction and Placenta (IX SLIMP); 2022

Objectives: Magnesium sulfate (MgSO4) is the drug of choice to prevent seizures in preeclamptic pregnant women, but its mechanism of action at the molecular level still is an enigma. We aimed to evaluate the effect of MgSO4 on AQP9 expression and function in human trophoblasts exposed to nitrative stress.Methods: This study was approved by the ethics committee of the Hospital Nacional Dr. Prof. A. Posadas. Placental explants were cultured in complete DMEM-F12 in the presence and absence of 200 mM peroxynitrite (ONOO-) to induce oxidative and nitrative stress. 4 mM MgSO4 was added to neutralize the effect of ONOO-. Lipid peroxidation (TBARS), superoxide dismutase 1 (SOD-1), and catalase expressions were analyzed. Water uptake and molecular expression of AQP9 were also studied.Results: Induction of oxidative and nitrative stress increased AQP9 protein a 75.42 ± 0.75% compared to control (n=7; p