Antiproliferative and anti-invasive effects of Vinorelbine in transitional cell carcinoma of bladder.

BONFIL, RD, RUSSO, DM, Y SCHMILOVICH, AJ.

Toronto, Canada

Congreso; 86thAnnual Meeting of the American Association for Cancer Research.; 1995

American Association for Cancer Research.

2083 Antiproliferative and anti-invasive effects of vinorelbine in transitional cell carcinoma of the bladder. Bonfil, R.D., Russo, D.M., and Schmilovich A.J. Lab. Fundación de Investigación del Cáncer, CEFYBO, and Lab. Rontag, Buenos Aires, Argentina. The semisynthetic Vinca alkaloid Vinorelbine (VNR) has demonstrated clinical activity against breast and non-small cell lung cancer, and lymphomas. The effect of VNR was studied in vitro in MB-49 a murine transitional cell carcinoma of the bladder (TCC). Cells were exposed t VNR for 3 h, followed by 48h recovery in drug-free medium. The number of viable cells was determined by the MTS colorimetric assay, observing a concentration dependent cell growth inhibition (IC50=40nM). The effect to VNR on MB-49 invasion was also evaluated. Cells exposed for 24h to VNR at a non-cytotoxic dose (10nM), and then tested for their ability to migrate across Matrigel-coated 8µm pore filters, showed significantly lower invasive abilities then non-treated cells (Mean number of cells ± SE: 2183 ±130 vs 5600 ± 625 respectively, p=0.0059, t-test ). These results would provide a rationale for the evaluation of intravesical chemotherapy with VNR in TCC experimental models.