The autophagy pathway status influenciates the extracellular vesicles profile released from pancreatic adenocarcinoma cells

DANIEL GRASSO; DANIELA L. PAPADEMETRIO; GIULIANA NARVÁEZ; MAXIMILIANO A. DIAZ; ELIDA ALVAREZ; MARIA NOÉ GARCIA

Mar del Plata

Congreso; Reunión Anual de Biociencias 2022; 2022

Sociedad Argentina de Investigación Clínica

Pancreatic ductal adenocarcinoma (PDAC) is a tough challenge due to its mortality and its extremely short life expectancy. Most cells release extracellular vesicles (EVs) that can transmit messages to distant target cells including effector actions. Autophagy is a homeostatic degradative program with implication in several cellular processes including, as it was suggested, the EVs biogenesis. This work was focused on the possible influences of the autophagy pathway over the EVs from PDAC cells. Immunofluorescence in PDAC cell line Panc-1 showed that the starvation (STV)-mediated induction of autophagy provokes a significant redistribution of the EVs markers CD81 and CD9 towards the plasma membrane where they colocalize partially. Moreover, STV induces a slight increase of extracellular signal for CD81 and CD9 (p