Peanut and maize growth stage affects the rhizobacterial community structure

ANZUAY MARIA SOLEDAD; PIN VISO, NATALIA; LILIANA MERCEDES LUDUEÑA; FEDERICO MORLA; -ANGELINI, JORGE,; TANIA TAURIAN

Congreso; XVI Symposium on Biological Nitrogen Fixation with Non- Legumes, together with the IV Latin-American workshop on PGPR and the XIX RELARE; 2018

Peanut (Arachis hypogaea L.) is an economicallyimportant leguminous crop in Argentina. The rotationof peanut with other crops, especially cereal crops iscrucial to obtain a good yield. In the agricultural areaof Argentina, maize (Zea mays L.) is the main cropused in rotation with peanuts. Rizosphere bacteriacan exert beneficial effects on plant growth grow anddevelopment by releasing nutrients into the rizosphere.This soil area is a versatile and dynamic ecologicalenvironment of intense microbe plant interactions andthe rhizobacterial communities can be affected by a wide range of factors including environmental factors such us soil type and plant species. Considering theimportance of peanut and maize crops, in the objective ofthis study was to analyze bacterial community structureassociated with the phenological stages of these plants.For this, a microcosm assay was performed using soilfrom the peanut growing area of Argentina as plantgrowth support. Unsterile soil was placed in plasticpots and previously peanut and maize disinfected seedswere sown. Plants were maintained under controlledenvironmental conditions during 120 and 100 days forpeanut and maize respectively. For bacterial rizhosphereanalysis, soil samples were taken at the beginning ofthe assay for both plants and at 60 and 120 days forpeanut and at 40 and 100 for maize. Soil DNA from thesesamples was extracted using a commercial kit. Bacterialcommunity structure of soil rizosphere was analyzed byhigh-throughput sequencing of 16S rRNA gene (IlluminaMiSeq platform). Results obtained indicated that the mostabundant phyla, in both peanut and maize rizospherewere Proteobacteria, Acidobacteria, Actinobacteria andBacteroidetes. Rhizobacterial community structures weredifferent depending on plant species and significantchanges were observed through plant growth stages atdiverse taxonomic levels (phylum, family and genus).The most significant differences were observed on therizosphere of late plant growth stages. In conclusion,the structure of the rhizospheric bacterial community isdynamic and is influenced by phenological plant growthstage.Keywords: bacterial community; plant development; IlluminaMiSeq platform.Financial Support: CONICET; SCyT-UNRC; ANPCyT.